Effects of ionizing radiation on expression of genes encoding cytoskeletal elements: Kinetics and dose effects

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Mol. Carcinog; (USA)

DOI:https://doi.org/10.1002/mc.2940030609· OSTI ID:6054072

Woloschak, G E; Shearin-Jones, P; Chang-Liu, C M ^[1]

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1. Argonne National Laboratory, IL (USA)

We examined the modulation in expression of genes encoding three cytoskeletal elements (beta-actin, gamma-actin, and alpha-tubulin) in Syrian hamster embryo (SHE) cells following exposure to ionizing radiations. Early-passage SHE cells were irradiated in plateau phase with various low doses (12-200 cGy) of neutrons, gamma-rays, or x-rays. RNA samples were prepared from cells at different times postexposure and were analyzed for levels of specific transcripts by northern blots. The results revealed that alpha-tubulin was induced by both high-linear energy of transfer (LET) (neutrons) and low-LET (gamma-rays and x-rays) radiations with similar kinetics. The peak in alpha-tubulin mRNA accumulation occurred between 1 and 3 h postexposure; for gamma-actin mRNA, accumulation was similarly induced. For both gamma-actin and alpha-tubulin, the higher the dose during the first hour postexposure (up to 200 cGy gamma-rays), the greater the level of mRNA induction. In contrast, mRNA specific for beta-actin showed decreased accumulation during the first hour following radiation exposure, and remained low up to 3 h postexposure. These results document the differential modulation of genes specific for cytoskeletal elements following radiation exposure. In addition, they demonstrate a decrease in the ratio of beta-actin:gamma-actin mRNA within the first 3 h following gamma-ray exposure. These changes in mRNA accumulation are similar to those reported in some transformed cell lines and in cells treated with tumor promoters, which suggests a role for changes in actin- and tubulin-mRNA expression in radiation-mediated transformation.

• OSTI ID: 6054072
• Journal Information: Mol. Carcinog; (USA), Journal Name: Mol. Carcinog; (USA) Vol. 3:6; ISSN 0899-1987; ISSN XY971
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTIN
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY TRANSFER
FERMIONS
GAMMA RADIATION
GENE REGULATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HADRONS
HAMSTERS
IONIZING RADIATIONS
LET
MAMMALS
MESSENGER-RNA
NEUTRONS
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
TRANSCRIPTION
VERTEBRATES
X RADIATION