Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
- Argonne National Lab., IL (United States)
Experiments were designed to examine the effects of radiation dose-rate and of the protein synthesis inhibitor cycloheximide on expression of cytoskeletal elements ({gamma}- and {beta}-actin and {alpha}-tubulin) and matrix elements (fibronectin) in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for JANUS fission-spectrum neutrons when comparing expression of either a-tubulin or fibronectin genes. Past work had already documented similar results for expression of actin transcripts. Cycloheximide, however, repressed accumulation of {alpha}-tubulin following exposure to high dose-rate neutrons or {gamma} rays; this did not occur following similar low dose-rate exposures. Cycloheximide did not affect accumulation of mRNA for actin genes. Cycloheximide abrogated the moderate induction of fibronectin-mRNA which occurred following exposure to {gamma} rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of {alpha}-tubulin and fibronectin mRNA accumulation following exposure to ionizing radiation and that the cellular/molecular response to low dose-rate neutrons may be different from the response to high dose-rate neutrons.
- Research Organization:
- Argonne National Lab., IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 10130291
- Report Number(s):
- ANL/CMB/PP--81485; ON: DE94007670
- Country of Publication:
- United States
- Language:
- English
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Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Technical Report
·
Thu Jun 01 00:00:00 EDT 1995
·
OSTI ID:70715
Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Technical Report
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Wed Dec 30 23:00:00 EST 1992
·
OSTI ID:10185724
Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Technical Report
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Sun May 01 00:00:00 EDT 1994
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OSTI ID:10148882
Related Subjects
560120
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTIN
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
COBALT 60
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
EMBRYONIC CELLS
ENZYME INDUCTION
FISSION NEUTRONS
GAMMA RADIATION
MESSENGER-RNA
PHOSPHORUS 32
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
TRACER TECHNIQUES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTIN
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
COBALT 60
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
EMBRYONIC CELLS
ENZYME INDUCTION
FISSION NEUTRONS
GAMMA RADIATION
MESSENGER-RNA
PHOSPHORUS 32
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
TRACER TECHNIQUES