Repair processes and radiation quality in neoplastic transformation of mammalian cells

Conference · Wed Aug 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

OSTI ID:6539800

Han, A; Hill, C K; Elkind, M M

Appropriate in vitro mammalian cell systems facilitate the study of cellular mechanisms of radiation oncogenesis. C3H 10T1/2 cells derived from mouse embryo have been used to study the effects of protracted irradiation on cell killing and incidence of neoplastic transformation. Protracted exposures of /sup 60/Co ..gamma.. rays or fission-spectrum neutrons (from the JANUS reactor at the Argonne National Laboratory) were given either at low dose rates or as multifractionated regimens using high dose rates. Dose protraction of ..gamma.. rays by multifractionation at a high dose rate (50 rad/min) results in appreciable reduction in cell killing and also significantly reduces the incidence of neoplastic transformation. Irradiation at a low dose rate has the same qualitative effect. In contrast, protracted exposures of fission-spectrum neutrons, given at a low dose rate (0.086 or 0.43 rad/min), result in significant enhancement of the frequency of transformation in the dose region of up to 80 rad. However, the survival of cells is essentially the same as that for the same single dose of neutrons given at a high dose rate. These observations are consistent with net ''error-free'' repair of transformational damage following protracted exposure of a low LET radiation and possibly a net ''error-prone'' repair of transformational damage after protracted irradiations with fission-spectrum neutrons.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6539800
• Report Number(s): CONF-830220-
• Conference Information: Journal Name: Radiat. Res.; (United States) Journal Volume: 99:2
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKOXY RADICALS
ALKYL RADICALS
ANNEALING
CARBOHYDRATES
DATA
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
ENDOR
EXPERIMENTAL DATA
HEAT TREATMENTS
HEXOSES
INFORMATION
IONIZING RADIATIONS
MAGNETIC RESONANCE
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATIONS
RADICALS
RESONANCE
SACCHARIDES
STOICHIOMETRY
X RADIATION