Isolation of uv-sensitive variants of CHO-Kl by nylon cloth replicaplating

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · Somatic Cell Genet.; (United States)

DOI:https://doi.org/10.1007/BF01542971· OSTI ID:6203552

Stamato, T D; Waldren, C A

Techniques are described which permit the identification and isolation of uv-sensitive variants from mutagenized populations of Chinese hamster ovary (CHO) cells. Identification is based on the observation that within two days after receiving a dose of approximately 240 ergs/mm/sup 2/ of uv irradiation, most of the cells in a colony of CHO detach from the surface of a plastic tissue culture dish. At a lower dose of uv, which does not kill or detach a significant number of parental cells, uv-sensitive colonies are killed and become detached. Thus a clear plaque is produced in a lawn of unirradiated parental cells, marking the site occupied by a sensitive colony. Live cells from such sensitive colonies have been recovered from a nylon cloth replica prepared prior to irradiation and characterized. One uv-sensitive variant (CHO-UV-1) is indistinguishable from parental cells in x-ray resistance, chromosome number, generation time, and duration of the phases of the cell cycle.

• Research Organization: Univ. of Colorado, Denver
• OSTI ID: 6203552
• Journal Information: Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 3:4; ISSN SCEGD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
CHROMOSOMES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
HAMSTERS
IONIZING RADIATIONS
MAMMALS
MUTANTS
NYLON
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYAMIDES
POLYMERS
RADIATION EFFECTS
RADIATIONS
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION