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Isolation of cell cycle-dependent gamma ray-sensitive Chinese hamster ovary cell

Journal Article · · Somatic Cell Genet.; (United States)
DOI:https://doi.org/10.1007/BF01543175· OSTI ID:5849707
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A technique for the isolation of gamma ray-sensitive Chinese hamster ovary (CHO) cell mutants is described, which uses nylon cloth replica plating and photography with dark-field illumination to directly monitor colonies for growth after gamma irradiation. Two gamma ray-sensitive mutants were isolated using this method. One of these cells (XR-1) had a two-slope survival curve: an initial steep slope and then a flattening of the curve at about 10% survival. Subsequently, it was found that this cell is sensitive to gamma irradiation in G1, early S, and late G2 phases of the cell cycle, whereas in the resistant phase (late S phase) its survival approaches that of the parental cells. The D37 in the sensitive G1 period is approximately 30 rads, compared with 300 rads of the parental cell. This mutant cell is also sensitive to killing by the DNA breaking agent, bleomycin, but is relatively insensitive to UV light and ethyl methane sulfonate, suggesting that the defect is specific for agents that produce DNA strand breakage.
Research Organization:
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania
OSTI ID:
5849707
Journal Information:
Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 9:2; ISSN SCEGD
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BLEOMYCIN
CELL CYCLE
CHO CELLS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
EMS
ESTERS
GAMMA RADIATION
IONIZING RADIATIONS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
SEPARATION PROCESSES
STRAND BREAKS
SULFONIC ACID ESTERS