Isolation and characterization of Chinese hamster ovary cell lines sensitive to mitomycin C and bleomycin

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Cancer Res.; (United States)

OSTI ID:6232003

Robson, C N; Harris, A L; Hickson, I D

Seven Chinese hamster ovary K1 cell lines exhibiting sensitivity to anticancer drugs have been isolated by a replica-plating technique. Five of the mutants are hypersensitive to the DNA cross-linking agent mitomycin C. Of these, one is also appreciably sensitive to UV light. Significant variations in their cross-sensitivity to cis-platinum(II) diammine dichloride, chlorambucil, and Adriamycin have also been observed. Two additional mutants have been isolated on the basis of sensitivity to the radiomimetic agent bleomycin. One of these shows greater than 6-fold sensitivity to bleomycin, while the other is approximately 14 times more sensitive than the parental strain to bleomycin and is also hypersensitive to a number of other DNA-damaging agents, including cis-platinum(II) diammine dichloride, chlorambucil, X-rays, and UV light. Both bleomycin-sensitive mutants also exhibit some degree of sensitivity to Adriamycin. In all cases, the cell lines have been grown in continuous culture for 3 months without evidence of reversion and should act as suitable recipients in DNA transfection experiments aimed at identifying human DNA repair genes.

• Research Organization: Univ. of Newcastle upon Tyne, England
• OSTI ID: 6232003
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States); ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
CHO CELLS
DNA REPAIR
DOXORUBICIN
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
IONIZING RADIATIONS
MAMMALS
MITOMYCIN
MUTANTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION