Mutation and repair in an ultraviolet-sensitive Chinese hamster ovary cell line
Thesis/Dissertation
·
OSTI ID:5020005
An ultraviolet (UV) light-sensitive mutant of Chinese hamster ovary cells (CHO) has been isolated and characterized with respect to a number of post-irradiation responses. The UV-sensitive mutant, termed 43-3B, was isolated by replica plating of mutagenized CHO cells, followed by a challenge with UV radiation. 43-3B has the same growth rate and chromosome number as the wild-type CHO-9. 43-3B is hypersensitive to the lethal effects of UV light (D/sub 0/ of 0.3 J/m/sup 2/ as compared to 3.2 J/m/sup 2/ for the wild-type). A marked UV-hypermutability is observed in 43-3B as compared to the wild-type. The UV-sensitive mutant is also sensitive to killing by simulated solar light, although the D/sub 0/ ratio is not as great as for germicidal UV. A much reduced ability of recover control rates of semiconservative DNA synthesis after UV irradiation was observed in the repair-deficient 43-3B cell line, suggesting that the removal of UV-induced replication blocks by excision repair is the most important factor in allowing recovery of UV-inhibited DNA synthesis. Recovery of colony-forming ability between fractionated UV exposures was observed in the wild-type CHO-9, but little recovery was seen in 43-3B. This indicates that excision repair capability can also be important in split-fluence recovery.
- Research Organization:
- California Univ., Berkeley (USA)
- OSTI ID:
- 5020005
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL KILLING
CHO CELLS
COLONY FORMATION
COMPARATIVE EVALUATIONS
DATA
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
INFORMATION
INHIBITION
MUTAGENESIS
MUTANTS
NUCLEIC ACID REPLICATION
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL KILLING
CHO CELLS
COLONY FORMATION
COMPARATIVE EVALUATIONS
DATA
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
INFORMATION
INHIBITION
MUTAGENESIS
MUTANTS
NUCLEIC ACID REPLICATION
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
ULTRAVIOLET RADIATION