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Chinese hamster ovary mutant UV-1 is hypomutable and defective in a postreplication recovery process

Journal Article · · Somatic Cell Genet.; (United States)
DOI:https://doi.org/10.1007/BF01538856· OSTI ID:5636963
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CHO-UV-1 is a mutant of the Chinese hamster cell CHO-K1 hypersensitive to killing by ultraviolet light but with normal resistance to X-ray. It is also hypersensitive to killing by ethyl methane sulfonate. Hybrid clones formed bu fusing UV-1 and Chinese hamster lung cells display the normal ultraviolet resistance of the latter. The sensitive phenotype behaves, therefore, in a genetically recessive manner. Ultraviolet sensitivity of UV-1 is not associated with a deficiency in excision repair. Alkaline sucrose gradient sedimentation analysis of nascent DNA from ultraviolet-irradiated cells reveals that UV-1 is, however, markedly deficient in postreplication recovery. Furthermore, UV-1 has a lower rate of induced mutation to 6-thioguanine resistance than does the parental cell when treated with ultraviolet light or ethyl methane sulfonate. These results suggest that the phenotype of UV-1 is due to a mutation in a form of postreplication recovery which in normal cells is error prone.
Research Organization:
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania
OSTI ID:
5636963
Journal Information:
Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 7:3; ISSN SCEGD
Country of Publication:
United States
Language:
English

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Related Subjects

550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HAMSTERS
MAMMALS
MUTANTS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHENOTYPE
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES