Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

DNA repair-deficient Chinese hamster ovary cells exhibiting differential sensitivity to. gamma. rays under aerobic and hypoxic conditions

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576512· OSTI ID:5905015
;

The survival of the wild-type parent and two mutant lines of Chinese hamster cells, known to be defective in DNA repair, has been determined as a function of exposure to ..gamma.. rays under aerobic and hypoxic conditions. When compared to the wild-type line, one of the mutants selected for sensitivity to ethyl methyl sulfonate (EMS), and known to be defective in the repair of DNA strand breaks, exhibits a markedly enhanced sensitivity to aerobic irradiation but a reduced enhancement to hypoxic irradiation and thus an enhanced oxygen enhancement ratio (OER). In contrast, the other line, known to be defective in the incision step of excision repair, exhibits the reverse pattern of sensitivity and hence a reduced OER. The results are compared to findings in bacterial mutants and cells obtained from ataxia telangiectasia (AT) patients and heterozygotes.

Research Organization:
Univ. of Toronto, Ontario
OSTI ID:
5905015
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:3; ISSN RAREA
Country of Publication:
United States
Language:
English

Similar Records

Resistance of hypoxic cells to ionizing radiation is influenced by homologous recombination status
Journal Article · Tue Jan 31 23:00:00 EST 2006 · International Journal of Radiation Oncology, Biology and Physics · OSTI ID:20793318
N-ethylmaleimide sensitization of x-irradiated hypoxic Chinese hamster cells
Journal Article · Fri Jul 01 00:00:00 EDT 1977 · Radiat. Res.; (United States) · OSTI ID:7142800
Mutation and repair in an ultraviolet-sensitive Chinese hamster ovary cell line
Technical Report · Sat Oct 31 23:00:00 EST 1981 · OSTI ID:5174373

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL KILLING
CHO CELLS
COBALT 60
COBALT ISOTOPES
DNA REPAIR
ELECTROMAGNETIC RADIATION
EMS
ESTERS
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MUTAGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN ENHANCEMENT RATIO
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
STRAND BREAKS
SULFONIC ACID ESTERS
SURVIVAL CURVES
YEARS LIVING RADIOISOTOPES