Premature chromosome condensation following x irradiation of mammalian cells: expression time and dose-response. [Chinese hamster ovary cells]

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575032· OSTI ID:5558111

Griffiths, T D; Carpenter, J G

Premature chromosome condensation (PCC) in Chinese hamster ovary (CHO) cells following exposure to 300-kVp x rays was first detected in the mitosis that followed the second postirradiation S phase. Thus, cells irradiated in G1 first expressed PCC at the second postirradiation mitosis while cells irradiated in G2 did not express PCC until the third postirradiation mitosis. Cells irradiated in the S phase expressed PCC at the second postirradiation mitosis with a frequency that was related to the position of the cells in the S phase at the time of exposure, cells in the first half of the S phase (at the time of exposure) showing a higher frequency than cells positioned in the second half. Thus, DNA replication during the first postirradiation S phase may be involved in the processing of lesions that eventually give rise to PCC. For cells in G1 at the time of exposure, the D/sub o/ for PCC expression at the second postirradiation mitosis was around 825 rad, indicating that PCC may play only a minor role in x-ray-induced cell killing. Autoradiographic analysis indicated approximately 50% of the PCC patches scored were replicating DNA at the time condensation was attempted. Daughter cells derived from such cells would suffer loss of genetic material.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 5558111
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 19:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DATA
DATA FORMS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
IN VITRO
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MAMMALS
MUTATIONS
NUCLEIC ACID REPLICATION
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
TIME DEPENDENCE
VERTEBRATES
X RADIATION