Radiation-induced cellular reproductive death and chromosome aberrations. [. gamma. rays; V79B Chinese hamster cells in synchronous cultures]

Journal Article · Fri Dec 01 04:00:00 EST 1978 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574806· OSTI ID:6530162

Bedford, J S ^[1]; Mitchell, J B; Griggs, H G; Bender, M A

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1. Colorado State Univ., Fort Collins

If a major mode of cell killing by ionizing radiation is the death of cells containing visible chromosomal aberrations, as for example from anaphase-bridge formation at mitosis, then cells bearing such aberrations should be selectively eliminated from the population, resulting in an increased survival potential for the population remaining at each succeeding cell generation. Using synchronized V79B Chinese hamster cells, we measured the aberration frequency and the colony-forming ability of mitotic cells at each of the first three generations following irradiation in G1. Cells were resynchronized by mechanial harvest at each succeeding mitosis after irradiation in order to avoid mixing of generations in the cell population at later sampling times. As anticipated, the chromosome aberration frequencies decreased markedly from the first to the second and from the second to the third mitosis. The surviving fraction, however, was virtually the same for plating assays carried out immediately after irradiation, at the first, or at the second mitosis. The surviving fraction was significantly higher for cells reaching the third postirradiation mitosis. Survival and aberration frequencies were assayed again at approximately the fourteenth postirradiation division, by which time the irradiated and control populations were not significantly different.

• OSTI ID: 6530162
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 76:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CELL DIVISION
CHROMOSOMAL ABERRATIONS
COBALT 60
COBALT ISOTOPES
CORRELATIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MITOSIS
MUTATION FREQUENCY
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY
SURVIVAL CURVES
SYNCHRONOUS CULTURES
YEARS LIVING RADIOISOTOPES