Growth and biochemical characteristics of a detachment variant of CHO cells
Journal Article
·
· J. Cell. Physiol.; (United States)
A variant subline of Chinese hamster cells (line CHO) was isolated that had increased resistance to detachment from the substratum. Comparisons between parental and variant cells of the complex carbohydrates liberated during trypsin detachment showed that the variant cells synthesized little or no hyaluronic acid. These cells also had reduced amounts of other complex carbohydrates in the cell periphery. However, parental and variant cells did not differ in morphology, growth control, or cyclic AMP concentration. Profound changes in the physical and chemical nature of the cell periphery, in themselves, evidently are insufficient to cause changes in many aspects of cell behavior.
- Research Organization:
- Los Alamos Scientific Lab., NM
- OSTI ID:
- 7304985
- Journal Information:
- J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 90:3; ISSN JCLLA
- Country of Publication:
- United States
- Language:
- English
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