Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Detachment variants of Chinese hamster cells. Hyaluronic acid as a modulator of cell detachment

Journal Article · · Exp. Cell Res.; (United States)
; ;
Variants of the Chinese hamster cell line CHO have been isolated and characterized with respect to attachment and trypsin- or EGTA-mediated detachment kinetics, cell morphologies, and the complex carbohydrates (labeled with (/sup 3/H)glucosamine) of the cell surface. The variant which was more readily detached from the substratum exhibited a more rounded cell shape and had three times more label as hyaluronic acid on the cell surface than the parental cell. The slowly detaching variant had a morphology similar to the parental cell but only half the radioactivity ascribable to hyaluronic acid. Endogenous levels of cAMP were unaltered in the variants. Exogenous db-cAMP caused the cells to elongate and flatten but did not alter the characteristic detachment kinetics. The role of hyaluronic acid as a modulator of the cell substratum interface is discussed.
Research Organization:
Los Alamos Scientific Lab., NM
OSTI ID:
6503128
Journal Information:
Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 119; ISSN ECREA
Country of Publication:
United States
Language:
English

Similar Records

Growth and biochemical characteristics of a detachment variant of CHO cells
Journal Article · Mon Feb 28 23:00:00 EST 1977 · J. Cell. Physiol.; (United States) · OSTI ID:7304985
Growth-related variations in the glycosaminoglycan synthesis of ultraviolet light-induced murine cutaneous fibrosarcoma cells
Journal Article · Thu Aug 01 00:00:00 EDT 1985 · Lab. Invest.; (United States) · OSTI ID:5017382
Action of cyclic adenosine 3':5'-phosphate in Chinese hamster ovary cells
Conference · Tue Dec 31 23:00:00 EST 1974 · OSTI ID:7368372

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHELATING AGENTS
EGTA
ENZYMES
FEMALE GENITALS
GENETIC VARIABILITY
GLUCOSAMINE
GLYCOLS
GONADS
HAMSTERS
HEXOSAMINES
HEXOSES
HYALURONIC ACID
HYDROGEN ISOTOPES
HYDROLASES
HYDROXY COMPOUNDS
INTERFACES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MEMBRANES
MONOSACCHARIDES
MORPHOLOGY
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
PEPTIDE HYDROLASES
POLYSACCHARIDES
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SERINE PROTEINASES
TRITIUM
TRYPSIN
VERTEBRATES
YEARS LIVING RADIOISOTOPES