Growth-related variations in the glycosaminoglycan synthesis of ultraviolet light-induced murine cutaneous fibrosarcoma cells
Glycosaminoglycan synthesis was studied in cell populations of ultraviolet light-induced murine cutaneous fibrosarcoma cells under conditions of varying growth rates in vitro. After labeling with the precursors, /sup 3/H-glucosamine and /sup 35/SO/sub 4/, sulfated glycosaminoglycans recoverable by direct proteolysis of the culture monolayers increased approximately 5-fold on a per cell basis from sparsely populated, exponential cell cultures (greater than 85% of cells in S, G2, or M phases) to stationary cultures inhibited by high cell density (greater than 50% of cells in G1). Within this cell surface-associated material, the relative ratio of heparan sulfate to the chondroitin sulfates was approximately 60/40% under conditions of exponential growth; in the growth-arrested cultures, the reverse ratio was found. The substratum attached material, obtained from the flask surface after ethyl glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA)-mediated detachment of the monolayers, contained relatively more hyaluronic acid, heparan sulfate, and chondroitin sulfates in the most actively proliferating cultures compared with the growth-inhibited cell populations. Furthermore, heparan sulfate and the chondroitin sulfates, which were enriched in the substratum material and in the cell pellet of exponential cultures, showed a relative shift to the cell surface-associated compartment (releasable by mild trypsinization after EGTA-mediated cell detachment) and to the compartment loosely associated with the pericellular matrix (i.e., released into the supernatant during detachment of the monolayers in the presence of EGTA).
- Research Organization:
- Univ. of Utah School of Medicine, Salt Lake City
- OSTI ID:
- 5017382
- Journal Information:
- Lab. Invest.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
FIBROSARCOMAS
RADIOINDUCTION
GLUCOSAMINE
BIOSYNTHESIS
TUMOR CELLS
GROWTH
BIOCHEMICAL REACTION KINETICS
CELL DIVISION
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
EGTA
HYALURONIC ACID
IN VITRO
LABELLED COMPOUNDS
MICE
PRECURSOR
SULFATES
SULFUR 35
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ALCOHOLS
AMINES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
GLYCOLS
HEXOSAMINES
HEXOSES
HYDROXY COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SARCOMAS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550201 - Biochemistry- Tracer Techniques