Isolation of Chinese hamster ovary cells with reduced unscheduled DNA synthesis after UV irradiation
Journal Article
·
· Somatic Cell Genet.; (United States)
A simple procedure has been worked out to obtain UV-sensitive mutants of Chinese hamster ovary (CHO) cells. In this procedure, conventional mutagenesis is followed by BrdU--light treatment to enrich the population for UV-sensitive cells. Colonies that are allowed to form subsequently are duplicated by replica plating and screened on the master plate for their UV sensitivity and their capacity to carry out UV-induced DNA repair synthesis. Putative mutants are isolated from the replica. With this combination of methods, we succeeded in isolating CHO mutants with an 85-95% reduced level of UV-induced DNA synthesis in combination with an increased UV sensitivity.
- Research Organization:
- Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands
- OSTI ID:
- 6530249
- Journal Information:
- Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 8:5; ISSN SCEGD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BODY
CELL CULTURES
DNA
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
GONADS
HAMSTERS
MAMMALS
MUTAGENESIS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BODY
CELL CULTURES
DNA
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
GONADS
HAMSTERS
MAMMALS
MUTAGENESIS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES