Bacterial clearance in the intact and regenerating liver

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · J. Pediatr. Surg.; (United States)

DOI:https://doi.org/10.1016/S0022-3468(85)80211-6· OSTI ID:6179419

Gross, K; Katz, S; Dunn, S P; Cikrit, D; Rosenthal, R; Grosfeld, J L

The Kupffer cells in the liver play an important role in reticuloendothelial system (RES) function by clearing particulate matter and bacteria from the blood stream. While hepatocyte regeneration and function have been extensively studied following partial hepatectomy, little information is available concerning RES function in the regenerating liver. This study investigates hepatic RES function by evaluating bacterial clearance (live E. coli) in the intact and regenerating liver. Thirty-four young male Sprague Dawley rats were studied. Twenty-two animals underwent a standard 70% partial hepatectomy using ligature technique and 12 had a sham operation. Both groups of rats received 10(9) organism of TVS labeled E coli, intravenously at 24 hours, 72 hours, 2 1/2 weeks, and 6 weeks postoperatively. Rats were killed 10 minutes following injection and liver, lung, spleen, and kidney harvested, fixed, and radioactivity was determined using a scintillation spectrometer interfaced with a micro-computer counting the TVS radiolabel. The total organ count of trapped bacteria in liver in partially hepatectomized rats was lower than intact controls at 24 hours, but was similar at 72 hours, 2 1/2 weeks, and 6 weeks. Partial hepatectomy increased the amount of bacterial trapping in the lung at 24 hours and 72 hours and returned to normal at 2 1/2 weeks and 6 weeks. Splenic activity was increased following hepatectomy at 2 1/2 weeks. Renal clearance was increased at 72 hours and 2 1/2 weeks.

• Research Organization: James Whitcomb Riley Hospital for Children, Indianapolis, IN
• OSTI ID: 6179419
• Journal Information: J. Pediatr. Surg.; (United States), Journal Name: J. Pediatr. Surg.; (United States) Vol. 4; ISSN JPDSA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD-PLASMA CLEARANCE
BODY
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GLANDS
HEPATECTOMY
ISOTOPES
LIGHT NUCLEI
LIVER
LUNGS
MAMMALS
MEDICINE
MICROORGANISMS
NUCLEI
ORGANS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RETICULOENDOTHELIAL SYSTEM
RODENTS
SCINTILLATION COUNTING
SPECTROPHOTOMETRY
SPLEEN
SULFUR 35
SULFUR ISOTOPES
SURGERY
TISSUES
VERTEBRATES