Development of more efficacious Tc-99m organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1984-April 30, 1985

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6168399

Heineman, W R

Anion exchange high performance liquid chromatography (HPLC) has been used to separate components of /sup 99m/Tc(NaBH/sub 4/) - DMAD formulations prepared as a function of pH, the presence or absence of air, and the time post reaction. The formulation pH is an effective variable for controlling the generation and interconversion of /sup 99m/Tc-DMAD components, and for allowing the preparation of large quantities of specific /sup 99m/Tc-DMAD complexes for biological evaluation. Individual components of /sup 99m/Tc(NaBH/sub 4/)-DMAD mixtures, separated by anion exchange HPLC, have been evaluated as skeletal imaging agents in rats. Biodistribution data show that the evaluated exhibit markedly different bone uptake and soft tissue localization. Comparison of the Tc(NaBH/sub 4/)-DMAD complex exhibiting the most favorable biological distribution with Osteoscan using an osteogenic rat model showed the DMAD complex to be slightly more efficacious. The total concentration of technetium in the eluants of commercially available /sup 99/Mo//sup 99m/Tc radionuclide generators has been determined by five different analytical procedures using four analytical techniques. Liquid scintillation beta counting, gas flow proportional beta counting, ionization chamber beta counting and high performance liquid chromatography with ultraviolet detection were used to track /sup 99/TcO/sub 4//sup -/ in the eluants of clinically used generators from each of the four domestic (USA) manufactures. The relative merits and disadvantages of the five procedures for the analysis of total technetium in the eluants of commercially available generators have been evaluated. 9 references, 3 figures.

• Research Organization: Cincinnati Univ., OH (USA). Dept. of Chemistry
• DOE Contract Number: AC02-80EV10380
• OSTI ID: 6168399
• Report Number(s): DOE/EV/10380-5; ON: DE85006612
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
400400 -- Electrochemistry
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTROCHEMISTRY
ESTERS
EVALUATION
EVEN-ODD NUCLEI
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ION EXCHANGE CHROMATOGRAPHY
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MOLYBDENUM 99
MOLYBDENUM ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHONIC ACID ESTERS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPE GENERATORS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
REDOX REACTIONS
RODENTS
SEPARATION PROCESSES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
VERTEBRATES
YEARS LIVING RADIOISOTOPES