Development of more efficacious Tc-99m organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1982-April 30, 1983

Technical Report · Mon Nov 01 04:00:00 EST 1982

OSTI ID:6607725

Heineman, W R; Deutsch, E A

A procedure based on high performance liquid chromatography (HPLC) has been developed for separating individual Tc-diphosphonate complexes in skeletal-imaging radiopharmaceuticals prepared by reduction of TcO/sub 4//sup -/ with NaBH/sub 4/ in the presence of methylene diphosphonate (MDP). Seven different Tc-MDP complexes have been detected and isolated in pure form. Significant differences in skeletal uptake and blood clearance are exhibited by the three complexes tested thus far by biodistribution studies in test animals. The relative quantities of these complexes present in a radiopharmaceutical preparation are dramatically influenced by the pH of the reaction mixture. Thus, control of pH is a simple means of forming in high yield the single, most efficacious Tc-MDP complex for skeletal imaginG. An HPLC method with electrochemical detection has been developed for the analytical determination of total TcO/sub 4//sup -/ (/sup 99m/Tc + /sup 99/Tc). Concentrations of TcO/sub 4//sup -/ as low as 9 x 10/sup -9/ M can be detected. The method is being evaluated for monitoring total TcO/sub 4//sup -/ in /sup 99/Mo//sup 99m/Tc generators.

• Research Organization: Cincinnati Univ., OH (USA)
• DOE Contract Number: AC02-80EV10380
• OSTI ID: 6607725
• Report Number(s): DOE/EV/10380-3; ON: DE83002767
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL PREPARATION
CHROMATOGRAPHY
DRUGS
ESTERS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PERTECHNETATES
PH VALUE
PHOSPHONIC ACID ESTERS
QUANTITY RATIO
RADIOISOTOPES
RADIOPHARMACEUTICALS
SEPARATION PROCESSES
SYNTHESIS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES