Tritiated uracil, tritiated thymidine, and bromodeoxyuridine induced mutations in eucaryotic cells. [Saccharomyces cerevisiae, gamma radiation]
The induction of gene conversion at the ARG-4 locus in strain BZ34 of Saccharomyces cerevisiae was examined after the cells incorporated y-/sup 3/H uracil under optimum growth conditions for 16 hours, and then received damage at 4/sup 0/C from tritium decays at very low dose rates of 1.4 to 27.6 tritium decays per hour. The results were compared to the results of gene conversion induced by /sup 60/Co. The induction of resistance to 6TG in Chinese hamster ovary (CHO) cells has been studied after incorporation of /sup 3/H-methyl thymidine, 6-/sup 3/H-thymidine, and bromodeoxyuridine under several experimental conditions. The induction of mutations by incorporated 6-/sup 3/H-thymidine is about three times as effective as the induction of mutations by tritiated-methyl thymidine. These results suggest that the determination of the RBE for tritium decays in model eucaryotic systems like yeast and cultured Chinese hamster cells will be influenced by the precise experimental conditions employed. In particular, experiments with mammalian cells will be affected by hot times for mutagenesis in the cell cycle and hot positions within the DNA in the nucleus, and also by the position of tritium decay within the DNA-incorporated molecule.
- Research Organization:
- California Univ., Berkeley (USA). Lawrence Berkeley Lab.; California Univ., San Francisco (USA). Lab. of Radiobiology; Institut du Radium, 91 - Orsay (France)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6164908
- Report Number(s):
- LBL-8811; CONF-790325-4; IAEA-SM-237/39
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
BUDR
DATA
DATA FORMS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FUNGI
GAMMA RADIATION
GENES
GENETIC EFFECTS
GRAPHS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INFORMATION
IONIZING RADIATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTATIONS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RBE
RIBOSIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
THYMIDINE
TRITIUM
URACILS
YEARS LIVING RADIOISOTOPES
YEASTS