Differences in the ability of human lymphoblastoid lines to exclude bromodeoxyuridine and in their sensitivity to methyl methanesulfonate and to incorporated (/sup 3/H)thymidine

Journal Article · Thu Feb 01 04:00:00 EST 1979 · Cancer Res.; (United States)

OSTI ID:7056116

Higgins, N P; Strauss, B S

Six Burkitt's lymphoma-derived lines were more resistant to methyl methanesulfonate than were six lymphoblastoid lines derived by in vitro transformation as measured by cloning ability in soft agar. The differences were due to cell response rather than to reactivity. Lymphoblastoid lines were also more sensitive to (/sup 3/H)thymidine incorporated along with bromodeoxyuridine than were the lymphoma lines tested. All lines showed excision repair activity as measured by the benzoylated naphthoylated DEAE cellulose method, although there were quantitative differences in the activity of different lines. Most lymphoblastoid lines synthesize DNA of lower density when incubated in analog-containing medium due to their ability to discriminate against bromodeoxyuridine and to select for thymidine in a mixture. All the lymphoma cell lines tested were unable to discriminate between thymidine were unable to discriminate between thymidine and bromodeoxyuridine. The ability of lymphoma cells to produce large quantities of dense radioactive, doubly substituted DNA when incubated in medium with bromodeoxyuridine and thymidine is due to their inability to discriminate between nucleosides and to their resistance to incorporated (/sup 3/H)thymidine.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 7056116
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
BUDR
CARBON 14 COMPOUNDS
CELL CULTURES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DATA
DISEASES
DNA
DRUGS
ESTERS
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INFORMATION
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOMAS
MAMMALS
MAN
MATERIALS
METHYL METHANESULFONATE
MUTAGENS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
SENSITIVITY
SOMATIC CELLS
SULFONIC ACID ESTERS
SURVIVAL CURVES
SYNTHESIS
THYMIDINE
TRITIUM COMPOUNDS
UPTAKE
URACILS
VERTEBRATES