Use of human lymphoblastoid cells for the estimation of environmental insults to DNA. Progress report, March 15, 1975--March 14, 1976. [Repair of DNA in cultured human peripheral blood lymphocytes]

Technical Report · Thu Jan 01 04:00:00 EST 1976

OSTI ID:7195154

Strauss, B S

During the past year this laboratory has been engaged in exploitation of the newly developed BND-cellulose (benzoylated naphthoylated DEAE cellulose) methodology for the measurement of DNA excision repair. We have demonstrated that peripheral blood lymphocytes are essentially excision repair deficient and that their capability for repair increases on development of a DNA synthetic apparatus. We have been able to separate DNA growing points from the bulk of the DNA and to show that in human lymphoma cells the mutagen methyl nitronitrosoguanidine induces about 4 times the repair activity in the growing point region as in the bulk of the DNA. Mutagens such as methyl methanesulfonate and acetoxyacetylaminofluorene induce equal repair activities in the two DNA regions. We have adapted our methodology to a batch procedure in order to facilitate its routine use. (auth)

• Research Organization: Chicago Univ., Ill. (USA)
• OSTI ID: 7195154
• Report Number(s): COO-2040-9
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
LEUKOCYTES
LYMPHOCYTES
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS