Use of human lymphoblastoid cells for the estimation of environmental insults to DNA. Progress report, March 15, 1976--June 30, 1977

Technical Report · Fri Jul 01 04:00:00 EDT 1977

OSTI ID:7316524

Strauss, B S

Benzoylated naphthoylated DEAE cellulose (BND cellulose) can be used for the detection of breaks in DNA since molecules with single strand interruptions adhere to the resin after shearing. Treatment with Sl nuclease reduces adherence by removing single stranded tails produced by the shear forces. Sl nuclease can also be added to the top of a neutral sucrose gradient to convert single stranded interruptions to double stranded breaks detectable on centrifugation. Using these methodologies, we have separated several carcinogens into those which induce numerous breaks into the DNA of human lymphoid cells and those inducing relatively few. Agents such as acetoxy acetylaminofluorene which are repaired by nucleotide excision repair produce few breaks in human lymphoid cells; agents such as methyl methanesulfonate, which induce base excision (apurinic) repair produce many breaks. Treatment with the antidiolepoxide of benzypyrene (anti-BPDE) does not induce numerous breaks, but DNA with aromatic hydrocarbon adducts will adhere to BND cellulose. Repair of anti-BPDE induced damage occurs less readily in complementation group C xeroderma lymphoblasts and is therefore likely to be by nucleotide excision. Repair functions are affected by the state of cell differentiation. Early embryonic chick retinocytes are able to repair, but this capability is lost on further development. Cultures of cells obtained from differentiated chick retinas regain the ability to replicate DNA and divide, but the repair function remains switched off.

• Research Organization: Chicago Univ., IL (USA)
• DOE Contract Number: EY-76-S-02-2040
• OSTI ID: 7316524
• Report Number(s): COO-2040-10
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL DIFFERENTIATION
CELLULOSE
CENTRIFUGATION
CHEMISTRY
CHICKENS
CONNECTIVE TISSUE CELLS
DNA
EMBRYOS
ENZYMES
FOWL
LEUKOCYTES
LYMPHOCYTES
NUCLEASES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PHOSPHOTRANSFERASES
POLYMERS
POLYSACCHARIDES
RECOVERY
REPAIR
RESINS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
VERTEBRATES