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Bromodeoxyuridine-induced mutations in synchronous Chinese hamster cells: temproal induction of 6-thioguanine and ouabain resistance during DNA replication

Journal Article · · Genetics; (United States)
OSTI ID:6470896
;

Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguanine (6TG) and ouabain resistance. No mutants above background were induced by exposure to BUdR in G1 and G2 cells, and very few mutants were induced in the latter part of the DNA synthesis period. The peak for the induction of 6TG resistance occurs at about two hr in the DNA synthesis period; one hour later there is a peak for the induction of ouabain resistance. Both peaks occur before the time of maximum incorporation of BUdR into DNA. These results suggest that the mutagenesis by BUdR is associated with at least two nuclear genes, which replicate at two hr and three hr in the DNA synthesis period.

Research Organization:
Lawrence Berkeley Lab., CA
OSTI ID:
6470896
Journal Information:
Genetics; (United States), Journal Name: Genetics; (United States) Vol. 90:2; ISSN GENTA
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BROMOURACILS
BUDR
CARBOHYDRATES
CARDIOTONICS
CELL CULTURES
CELL CYCLE
DNA
DRUGS
GENETIC EFFECTS
GLYCOSIDES
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PURINES
PYRIMIDINES
RIBOSIDES
RODENTS
STEROIDS
STROPHANTIN
SYNCHRONOUS CULTURES
SYNTHESIS
URACILS
VERTEBRATES