Mutation induction in synchronous hamster cells
Mutagenesis of synchronous Mutahinese hamster cells by 5-bromodeoxyuridine (BUdR) shows pronounced cell cycle dependency. Resistance to 6-thioguanine (6-TG) and ouabain are induced maximally by BUdR at different times early in the DNA synthesis period, suggesting that the genes coding for hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and the (Na/sup +/K/sup +/)-associated ATPase of the plasma membrane are replicated early in the DNA synthesis period. Although BUdR induces mutations in specific genes only when present during their replication, the rate of mutation induction is not linearly related to the amount of BUdR incorporated into DNA. The data show a BUdR concentration threshold for mutation induction, suggesting that BUdR exerts some deterimental allosteric effect on DNA synthesis enzymes.
- Research Organization:
- California Univ., Berkeley (USA). Lawrence Berkeley Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 7364254
- Report Number(s):
- LBL-4720
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
ATP-ASE
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BROMOURACILS
BUDR
CELL CULTURES
CELL CYCLE
DNA
DRUGS
ENZYMES
ESTERASES
GENES
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
MAMMALS
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PYRIMIDINES
RIBOSIDES
RODENTS
SYNCHRONOUS CULTURES
SYNTHESIS
TRANSFERASES
URACILS
VERTEBRATES