Ionizing radiation-induced 6-thioguanine-resistant clones in synchronous CHO cells
Journal Article
·
· Radiat. Res.; (United States)
When cultured Chinese hamster ovary (CHO) cells are exposed to acute doses of ionizing radiation at different times during the cell division cycle, there is a characteristic cell-cycle response for radiation-induced cell killing and induced resistance to 6-thio-guanine (6TG). For cell killing the sensitive periods of the cell cycle are the G1, G2, M, and early S periods, as others have reported. For mutation induction the sensitive stage is the G1 period with the maximum sensitivity near the boundary between the G1 and the S period. Cells appear to be very refractile to induction of 6TG resistance in other periods of the cell cycle. These results suggest that chromosomal rearrangements of the X chromosome are most likely to occur in the G1 period before the gene for hypoxanthine-guanine-phosphoribosyl-transferase replicates, most likely due to genetic recombination. Clones resistant to 6TG after exposure to x rays are most likely induced by a different mutagenic pathway than ones stimulated by ultraviolet (uv) or ethylnitrosourea treatments, since the mutation induction patterns in the cell cycle are quite different.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 5419203
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 81:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300 -- Cytology
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CELL KILLING
CHROMOSOMES
CLONE CELLS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MAMMALS
MUTATION FREQUENCY
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RODENTS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
THIOLS
TIME DEPENDENCE
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CELL KILLING
CHROMOSOMES
CLONE CELLS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MAMMALS
MUTATION FREQUENCY
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RODENTS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
THIOLS
TIME DEPENDENCE
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION