Comparison of radon-daughter-induced effects in repair-proficient and repair-deficient CHO cell lines

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

OSTI ID:6316243

Schwartz, J L; Shadley, J D; Atcher, R W; Tang, J; Whitlock, J L; Rotmensch, J ^[1]

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1. Argonne National Lab., IL (USA)

The radiobiological effects of the radon daughter 212Bi were investigated in the Chinese hamster ovary cell line AA8 and its radiosensitive derivative EM9. EM9 cells rejoin radiation-induced DNA strand breaks more slowly than do AA8 cells. Three endpoints were examined: cell killing, G2-induced chromosome aberration frequency, and mutation induction at the hypoxanthine (guanine) phosphoribosyltransferase (HGPRT) locus. Cells were exposed to the alpha-emitter 212Bi chelated to diethylenetriaminepentaacetic acid (212Bi-DTPA). As expected, 212Bi-DTPA was more effective than X-rays in producing cytotoxicity, chromosome aberrations, and gene mutations. The relative biological effectiveness (RBE) for all three endpoints ranged from about 2 for chromosome aberrations to 4.4 for mutation induction. EM9 was more sensitive than AA8 cells to the cytotoxic and clastogenic effects of both X-rays and 212Bi-DTPA, suggesting that the repair deficiency in EM9 cells affects response to low- and high-linear energy transfer (LET) radiation for these endpoints. There was no significant difference between these two cell lines in their mutagenic response to X-rays and AA8 was slightly more sensitive to the mutagenic effects of alpha radiation. These results suggest that alterations in DNA repair ability may affect response of cells to both low- and high-LET radiation-induced cytotoxicity and clastogenicity, but they appear to have little effect on gene mutation induction.

• OSTI ID: 6316243
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 16:3; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALPHA DECAY RADIOISOTOPES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BISMUTH 212
BISMUTH ISOTOPES
CARBOXYLIC ACIDS
CHELATING AGENTS
CHO CELLS
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
DNA
DNA REPAIR
DRUGS
DTPA
ELECTROMAGNETIC RADIATION
ENERGY TRANSFER
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
IONIZING RADIATIONS
ISOTOPES
LET
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RBE
RECOVERY
REPAIR
STRAND BREAKS
X RADIATION