Spectra of base substitution mutations induced in Escherichia coli by tritiated water and the decay of incorporated tritiated thymidine

Journal Article · Sat Dec 31 23:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576201· OSTI ID:5176801

Ise, T ^[1]; Kato, T; Glickman, B W

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1. Osaka Univ. Medical School, Japan

The biological effects of tritiated water and of (6-/sup 3/H)thymidine or (methyl-/sup 3/H)thymidine incorporated into DNA were compared with those induced by /sup 60/Co ..gamma.. rays. The killing efficiences of tritiated water and the tritium-labeled bases were very similar, between 1.8 and 2.0 in terms of the RBE of /sup 60/Co ..gamma.. rays when compared with absorbed dose to the bacterial nucleus. The frequency of His/sup +/ revertants induced by the decay of (6-/sup 3/H)thymidine was 3.5 times higher than that induced by (methyl-/sup 3/H)thymidine or tritiated water; these revertants were most often the result of A:T..-->..G:C transitions. In comparison, the other treatments efficiently induced both transitions and transversions. The mutational spectrum resulting from the decay of tritiated water was also determined in the lacI forward-mutagenesis system of Escherichia coli. Transitions predominated at the low dose (2.5 krad), while both transitions and transversions were recovered after a high dose (18 krad). These results are very similar to those observed with /sup 60/Co ..gamma.. rays and are consistent with the hypothesis that mutagenesis resulting from the decay of (6-/sup 3/H)thymidine is the result of a position effect, while mutagenesis resulting from the decay of (methyl-/sup 3/H)thymidine and tritiated water is due to ..beta..-particle ionization.

• OSTI ID: 5176801
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550701 -- Microbiology-- Tracer Techniques
560164* -- Radionuclide Effects-Internal Source-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHALCOGENIDES
COBALT 60
COBALT ISOTOPES
DNA
DOSES
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MUTANTS
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
RADIATION QUALITY
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RBE
REVERTANTS
RIBOSIDES
THYMIDINE
TRITIUM COMPOUNDS
TRITIUM OXIDES
YEARS LIVING RADIOISOTOPES