Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]
Actively growing V79 Chinese hamster cells, treated with anisotonic phosphate-buffered saline (PBS) after x irradiation, are more sensitive than cells treated with isotonic PBS or cells promptly incubated with complete medium immediately after irradiation. The sensitization of irradiated cells results from hypotonic as well as hypertonic NaCl concentrations in PBS, is strongly dependent on both temperature and time, and is mainly due to an increase in the final slope of the single-dose survival curve. After two x-ray dose fractions, the net response of cells sensitized after each fraction by anisotonic post-treatment is similar to that obtained for isotonically treated cells and indicates that sublethal damage repair is not influenced by the enhanced expression of lethal damage. Independence of the repair of damage which is potentially lethal from the repair of damage which is sublethal is further suggested by the more rapid rate of the former compared to that of the latter. The proposal is advanced that the enhanced expression of damage which, after x irradiation, can be shown to be potentially lethal results from a destabilization of the structural relationship between DNA and the nuclear envelope, and/or DNA and the nuclear protein matrix, as a consequence of osmotic changes produced by anisotonic treatment.
- Research Organization:
- Argonne National Lab., IL
- OSTI ID:
- 6138109
- Journal Information:
- Radiat. Res.; (United States), Vol. 77:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of caffeine on the expression of potentialy lethal and sublethal damage in. gamma. -irradiated cultured mammalian cells. [Human T-1 cells and Chinese hamster V79-79 cells]
Fixation of potentially lethal radiation damage in Chinese hamster cells by anisotonic solutions, polyamines, and DMSO
Related Subjects
ANIMAL CELLS
RADIOSENSITIVITY
DNA
BIOLOGICAL REPAIR
HYPERTONIC SOLUTIONS
RADIOSENSITIVITY EFFECTS
OSMOSIS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
ACUTE IRRADIATION
AGE DEPENDENCE
ANOXIA
BIOLOGICAL RADIATION EFFECTS
BUFFERS
CELL CULTURES
COMPARATIVE EVALUATIONS
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
GRAPHS
ISOLATED VALUES
LETHAL IRRADIATION
SODIUM CHLORIDES
SOLUTIONS
SUBLETHAL IRRADIATION
ULTRAVIOLET RADIATION
X RADIATION
ACUTE EXPOSURE
ALKALI METAL COMPOUNDS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CHLORIDES
CHLORINE COMPOUNDS
DATA
DATA FORMS
DISPERSIONS
ELECTROMAGNETIC RADIATION
HALIDES
HALOGEN COMPOUNDS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
MIXTURES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SODIUM COMPOUNDS
560121* - Radiation Effects on Cells- External Source- (-1987)