Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]

Journal Article · Thu Feb 01 04:00:00 EST 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575146· OSTI ID:6138109

Utsumi, H; Elkind, M M

Actively growing V79 Chinese hamster cells, treated with anisotonic phosphate-buffered saline (PBS) after x irradiation, are more sensitive than cells treated with isotonic PBS or cells promptly incubated with complete medium immediately after irradiation. The sensitization of irradiated cells results from hypotonic as well as hypertonic NaCl concentrations in PBS, is strongly dependent on both temperature and time, and is mainly due to an increase in the final slope of the single-dose survival curve. After two x-ray dose fractions, the net response of cells sensitized after each fraction by anisotonic post-treatment is similar to that obtained for isotonically treated cells and indicates that sublethal damage repair is not influenced by the enhanced expression of lethal damage. Independence of the repair of damage which is potentially lethal from the repair of damage which is sublethal is further suggested by the more rapid rate of the former compared to that of the latter. The proposal is advanced that the enhanced expression of damage which, after x irradiation, can be shown to be potentially lethal results from a destabilization of the structural relationship between DNA and the nuclear envelope, and/or DNA and the nuclear protein matrix, as a consequence of osmotic changes produced by anisotonic treatment.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 6138109
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 77:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
AGE DEPENDENCE
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANOXIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BUFFERS
CELL CULTURES
CHLORIDES
CHLORINE COMPOUNDS
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
GRAPHS
HALIDES
HALOGEN COMPOUNDS
HYPERTONIC SOLUTIONS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
LETHAL IRRADIATION
MIXTURES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OSMOSIS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SODIUM CHLORIDES
SODIUM COMPOUNDS
SOLUTIONS
SUBLETHAL IRRADIATION
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
ULTRAVIOLET RADIATION
X RADIATION