Potentially lethal and DNA radiation damage: similarities in inhibition of repair by medium containing D/sub 2/O and by hypertonic buffer
Journal Article
·
· Radiat. Res.; (United States)
Killing of log-phase V79 Chinese hamster cells by ionizing radiation is enhanced when cells are treated immediately after irradiation with anisotonic phosphate-buffered saline or with medium containing 90% D/sub 2/O. These treatments are not toxic to unirradiated cells. In medium containing D/sub 2/O, cells are unable to repair sublethal damage; however, repair occurs after a shift to normal medium. Thus, as is observed with anisotonic buffer, enhanced killing by medium containing D/sub 2/O does not prevent cells from repairing sublethal damage. Further aspects of the enhanced expression of potentially lethal damage by medium containing D/sub 2/O and by anisotonic buffer are also similar. For example, both are largely ineffective after treatments involving nonionizing radiation, and both treatments retard the repair of DNA breaks. From the foregoing, and the partial overlapping of enhanced killing due to medium containing D/sub 2/O and due to hypertonic saline, we infer that the latter treatments affect the repair, or reparability, of the same sensitive targets.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6853795
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 84:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Conference
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Tue Sep 01 00:00:00 EDT 1981
· Radiat. Res.; (United States)
·
OSTI ID:6394714
Potentially lethal damage versus sublethal damage: independent repair processes in actively growing Chinese hamster cells. [Effects of osmotic pressure differences due to NaCl concentrations in buffer solutions on sensitivity of cells to x rays]
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· Radiat. Res.; (United States)
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BUFFERS
CELL CULTURES
CELL KILLING
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXPERIMENTAL DATA
HAMSTERS
HEAVY WATER
HYDROGEN COMPOUNDS
INFORMATION
INHIBITION
IONIZING RADIATIONS
LETHAL DOSES
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
STRAND BREAKS
SURVIVAL CURVES
TIME DEPENDENCE
VERTEBRATES
WATER
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BUFFERS
CELL CULTURES
CELL KILLING
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXPERIMENTAL DATA
HAMSTERS
HEAVY WATER
HYDROGEN COMPOUNDS
INFORMATION
INHIBITION
IONIZING RADIATIONS
LETHAL DOSES
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
STRAND BREAKS
SURVIVAL CURVES
TIME DEPENDENCE
VERTEBRATES
WATER