Single-hit potentially lethal damage: evidence of its repair in mammalian cells

Conference · Tue Sep 01 04:00:00 EDT 1981 · Radiat. Res.; (United States)

OSTI ID:6394714

Utsumi, H; Hill, C K; Ben-Hur, E; Elkind, M M

Following mid to large doses of X rays, or of fission spectrum neutrons, the repair of potentially lethal damage in V79 Chinese hamster cells can be inhibited by anisotonic phosphate-buffered saline or by medium containing 90% D/sub 2/O. The foregoing post-treatments do not affect the viability of unirradiated cells. Using single synchronized cells irradiated in late S-phase, the most resistant phase of the cell cycle, repair of potentially lethal damage in late S-phase, the most resistant phase of the cell cycle, repair of potentially lethal damage in the single-hit, initially exponential, or small-dose part of the survival curve was examined. The use of synchronized cells avoids misinterpretations due to population heterogeneity. The slope of the small-dose, exponential region of the neutron survival curve is much steeper than that of the x-ray survival curve. Even so, it is demonstrated with post-treatments consisting of hypertonic phosphate-buffered saline, medium containing D/sub 2/O,adiation is connected with their proliferation but not with the migration out from lymphoid organs.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6394714
• Conference Information: Journal Name: Radiat. Res.; (United States) Journal Volume: 87:3
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALEPTICS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CAFFEINE
CENTRAL NERVOUS SYSTEM AGENTS
COMPARATIVE EVALUATIONS
DOSES
DRUGS
ELECTROMAGNETIC RADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
INJURIES
IONIZING RADIATIONS
MAMMALS
NEUTRON SPECTRA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
RODENTS
SPECTRA
SURVIVAL CURVES
VERTEBRATES
X RADIATION
XANTHINES