Detection of early changes in lung cell cytology by flow-systems analysis techniques. Progress report, January 1-June 30, 1979
This report summarizes results of ongoing experiments designed to develop automated flow-analysis assay methods for discerning damage to exfoliated respiratory tract cells in model test animals exposed by inhalation to physical and chemical agents associated with the production of synthetic fuels from oil shale and coal, the specific goal being the determination of atypical changes in exposed alveolar macrophages and epithelial cells. Animals were exposed to oil shale particles (raw and spent), silica, and polystyrene latex spheres via intratracheal instillation. Respiratory tract cells were obtained by lavaging the lungs with normal saline, stained with mithramycin for DNA content, and analyzed using flow cytometric analysis methods. In addition to measuring DNA content, differential and total cell counts were made on all samples analyzed. DNA content frequency distribution histograms and cytology showed definite atypical changes resulting from exposure to shale and silica particulates when compared to the controls. To continue development of fluorescence staining methods for measuring intracellular enzymes in alveolar macrophages, studies were initiated for determining ..beta..-glucuronidase using naphthol AS-BI-..beta..-d-glucuronic acid as a fluorogenic substrate. As this new technology becomes adapted to analyzing pulmonary macrophages and epithelial cells, the measurement of physical and biochemical properties as a function of exposure to particulate and gaseous toxic agents related to the production of synthetic fuels will be increased. This analytical approach is designed to assist in the establishment of future guideline for estimating the risks to exposed humans.
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6135406
- Report Number(s):
- LA-7983-PR
- Country of Publication:
- United States
- Language:
- English
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Flow cytometric methods for assaying damage to respiratory tract cells. [Hamsters exposed to agents associated with production of synthetic fuels from oil shale]
Detection of early changes in lung cell cytology by flow-systems analysis techniques. Progress report, January 1--December 31, 1978. [Resulting from exposure to toxic agents associated with production and use of synthetic fuels from oil shale and coal]
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANIMALS
BIOASSAY
BIOLOGY
BITUMINOUS MATERIALS
CARBONACEOUS MATERIALS
CHALCOGENIDES
COAL INDUSTRY
COLLOIDS
CYTOLOGY
DISPERSIONS
DNA
ENERGY SOURCES
FOSSIL FUELS
FUELS
GASEOUS WASTES
HAMSTERS
HAZARDS
HEALTH HAZARDS
INDUSTRY
MAMMALS
METAMORPHIC ROCKS
MINERALS
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
OIL SHALE INDUSTRY
OIL SHALES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RESPIRATORY SYSTEM
ROCKS
RODENTS
SHALES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOLS
SPENT SHALES
TOXICITY
VERTEBRATES
WASTES