Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters]
Flow cytometric techniques were used to measure the cytological and biochemical damage to respiratory tract cells in animals exposed to particulates. Hamsters were exposed to raw and spent oil shale particulates and silica by intratracheal instillation. Exfoliated lung cells were obtained by sacrificing the animals and lavaging the respiratory tract posterior to the trachea with saline. Cell samples were fixed in ethanol and stained with mithramycin for fluorescence analysis of DNA content. DNA content distributions from hamsters exposed to spent oil shale and silica particulates showed atypical changes 28 to 35 days later. Cell counts and total numbers of macrophages, leukocytes, and epithelial cells in the lavage fluid also showed marked changes related to time after exposure.
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5774426
- Report Number(s):
- LA-UR-79-2737; CONF-791002-1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DATA
DATA FORMS
DISEASES
DNA
EXPERIMENTAL DATA
FLUORESCENCE
HAMSTERS
INFORMATION
INTRATRACHEAL ADMINISTRATION
ISOLATED VALUES
LEUKOCYTES
LUMINESCENCE
MACROPHAGES
MAMMALS
METAMORPHIC ROCKS
MINERALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PARTICLES
PATHOLOGICAL CHANGES
PHAGOCYTES
QUANTITY RATIO
RESPIRATORY TRACT CELLS
ROCKS
RODENTS
SHALES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
SPENT SHALES
VERTEBRATES