Detection of early changes in lung cell cytology by flow-systems analysis techniques. Progress report, January 1--December 31, 1978. [Resulting from exposure to toxic agents associated with production and use of synthetic fuels from oil shale and coal]
This report summarizes ongoing experiments to develop cytological and biochemical indicators for measuring damage to respiratory tract cells of experimental animals exposed by inhalation to environmental toxic agents. The specific goal is to apply flow cytometric methods to analyze and detect changes in lung epithelium as a function of exposure to toxic agents associated with the production and utilization of synthetic fuels from oil shale and coal. During the past 6 months, hamsters were exposed to raw and spent oil shale particulates, silica dust, and ozone, and DNA content measurements were performed on lung cell samples. Although initial shale exposures did not yield the expected results, recent data show atypical changes in DNA content per cell distributions. Ozone exposures also were expanded to include DNA measurements and cytology, ranging up to 72 h postexposure. Progress was achieved in developing a new method for quantitating pulmonary macrophage phagocytosis in rats using micron-sized fluorescent spheres. New methods for determining alkaline phosphatase, DNA content, and protein also were under development. Plans are to continue developing cytological and biochemical markers for measuring atypical cellular changes, including macrophage function, and to emphasize exposing experimental animals to particulates and gaseous agents for studying dose-damage relationships.
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6272843
- Report Number(s):
- LA-7680-PR
- Country of Publication:
- United States
- Language:
- English
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Detection of early changes in lung cell cytology by flow-systems analysis techniques. Progress report, July 1--December 31, 1977
Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters]
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE PHOSPHATASE
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BITUMINOUS MATERIALS
CARBONACEOUS MATERIALS
CELL FLOW SYSTEMS
CHALCOGENIDES
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
CYTOLOGICAL TECHNIQUES
DIAGNOSTIC USES
DISEASES
DNA
DUSTS
ENERGY SOURCES
ENZYMES
ESTERASES
FOSSIL FUELS
FUELS
GUINEA PIGS
HYDROLASES
INHALATION
INTAKE
MACROPHAGES
MAMMALS
METAMORPHIC ROCKS
MINERALS
NUCLEIC ACIDS
OIL SHALES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
OZONE
PATHOLOGICAL CHANGES
PHAGOCYTES
PHAGOCYTOSIS
PHOSPHATASES
RESPIRATORY TRACT CELLS
ROCKS
RODENTS
SHALES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
SPENT SHALES
USES
VERTEBRATES