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Diethyldithiocarbamate enhancement of radiation and hyperthermic effects on Chinese hamster cells in vitro. [By inhibition of superoxide dismutase activity thus enhancing superoxide radical toxicity in the cells]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575161· OSTI ID:6082836
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To test the possibility of enhancing O/sub 2/- toxicity during radiation therapy and/or hyperthermia, Chinese hamster cells (Don) were treated with the drug diethyldithiocarbamate (DDC) in vitro. This drug has been shown to inhibit, both in vitro and in vivo, the enzyme superoxide dismutase (SOD) which is responsible for eliminating the 0/sub 2/- radical in cells. We observed that the cytocidal effect of DDC on Don cells is dependent on both DDC concentration and exposure time. After 8 to 10 days of incubation with 10/sup -9/ M DDC, no change in cell survival was noted; however, incubation with 10/sup -4/ M DDC produced a marked loss in colony formation. When DDC-treated cells were ..gamma..-irradiated, they did not survive as well as cells that were treated with either radiation or DDC alone. The combined effects of hyperthermia and DDC were dramatic. Cells treated 5 min at 43/sup 0/C with 10/sup -4/ M DDC or 10 min at 43/sup 0/C with 10/sup -5/ M DDC showed significant decrease in survival. These results suggest that DDC may be a potentially powerful sensitizing agent in tumor therapy, since there is increasing evidence that tumor cells have a lower level of superoxide dismutase.
Research Organization:
Tufts-New England Medical Center, Boston, MA
OSTI ID:
6082836
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 77:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CELL CULTURES
CHELATING AGENTS
COLONY FORMATION
DEDTC
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
GAMMA RADIATION
HYPERTHERMIA
INHIBITION
IONIZING RADIATIONS
IRRADIATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOSENSITIVITY EFFECTS
SUPEROXIDE RADICALS
SURVIVAL TIME