Combined effect of diethyldithiocarbamate (DCC) and modest hyperthermia on Chinese hamster (V79) cell survival and DNA strand break repair following photon irradiation

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90506-6· OSTI ID:6167021

Wagner, H Jr; Parkinson, D R; Madoc-Jones, H; Sternick, E S; Vrusho, K; Krasin, F

Previous studies from our institution have shown that DDC enhanced the effects of radiation and of hyperthermia treatment at 43/sup 0/C on the killing of Chinese hamster (DON) cells. The authors report studies on the combined effect seen at more modest temperatures (41/sup 0/C) which can be achieved in humans by whole body heating without the need for general anesthesia. Treatment of V79 cells with DDC for 60 minutes at 37/sup 0/C or 41/sup 0/C had minimal toxicity. When cells were irradiated with single doses up to 1000 rad, pre-incubation with DDC had no effect on cell survival at 37/sup 0/C, but markedly decreased survival at 41/sup 0/C. The mechanism of this increased cell killing is not known. They observed, however, that there is no repair of DNA single strand breaks in DNA from irradiated V79 cells previously held at 41/sup 0/C in the presence of DDC. Without DDC, repair of SSB was similar at 37/sup 0/C and 41/sup 0/C.

• Research Organization: Tufts-New England Medical Center, Boston, MA
• OSTI ID: 6167021
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:9; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY TEMPERATURE
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
CHELATING AGENTS
CONNECTIVE TISSUE CELLS
DEDTC
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
HAMSTERS
HYPERTHERMIA
IONIZING RADIATIONS
ISOTOPES
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
YEARS LIVING RADIOISOTOPES