Enhancement of oxygen toxicity by diethyldithiocarbamate on Chinese hamster and mouse cells

Conference · Sat Sep 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:5340059

Lin, P S ^[1]; Kwock, L; Lui, P; Hefter, K

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1. Tufts-New England Medical Center, Boston, MA

A Cu/sup + +/-Zn/sup + +/ containing enzyme, superoxide dismutase (SOD) converts the toxic O/sub 2//sup -/ radical to molecular O/sub 2/. This radical may play a significant role in the effects of radiation and anti-cancer agents on cellular damage and lethality. Based on the assumption that inhibition of SOD activity should lead to enhanced cellular damage and lethality by radiation and certain anti-cancer agents (i.e., bleomycin and adriamycin) we exposed Chinese hamster and mouse cells to a Cu/sup + +/-chelator diethyldithiocarbamate (DDC) to reduce intracellular SOD activity. Treated cells consistently showed a lower level of survival. Irradiation of DDC-treated cells reduced even further the relative survival of these cells. Cytological determination of DDC-treated cells showed two abnormalities, i.e., (1) distortion and swelling of mitochondria and (2) myelin-like figure formation. The results suggest that it may be potentially beneficial in tumor therapy to combine some conventional cancer therapy modalities with an agent such as DDC in lowering the cells' defense against O/sub 2//sup -/ toxicity.

• OSTI ID: 5340059
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:9
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL CULTURES
CHEMOTHERAPY
COBALT 60
COBALT ISOTOPES
CRYOGENIC FLUIDS
DATA
DATA FORMS
ELEMENTS
ENZYME INHIBITORS
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
FLUIDS
HAMSTERS
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOLATED VALUES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MITOCHONDRIA
NONMETALS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANOIDS
OXYGEN
RADIATION EFFECTS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RESPONSE MODIFYING FACTORS
RODENTS
SURVIVAL CURVES
THERAPY
TOXICITY
VERTEBRATES
YEARS LIVING RADIOISOTOPES