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Diethyldithiocarbamate concentration effects and interactions with other cytotoxic agents on Chinese hamster cells (V79)

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576702· OSTI ID:5406257
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A metal chelator, diethyldithiocarbamate (DDC) perturbs the chromosome condensation processes in dividing cells. The length of the metaphase chromosomes in Chinese hamster cells (V79) treated with 17.2 ..mu..g/ml of DDC for 2 hr is about half of that in untreated cells. However, concentrations of 1.7 ..mu..g or 172 ..mu..g/ml DDC apparently do not produce this effect. DDC at 17.2 ..mu..g/ml also disrupts spindle fibers. Bleomycin, but not mitomycin and cisplatin, added simultaneously with DDC can prevent the DDC effect on chromosomes. The cytotoxic effect of increasing concentrations of DDC can prevent the DDC effect on chromosomes. The cytotoxic effect of increasing concentrations of DDC to V79 cells incubated at 37/sup 0/C exhibits a similar biphasic response. This concentration biphasic toxic effect is not altered when the cells are treated with DDC in combination with radiation, heat, or other cytotoxic drugs. These observations suggest that the different effects of DDC concentrations on chromosome condensation should be considered as one important modification factor for DDC related toxicity.
Research Organization:
Tufts-New England Medical Center, Boston, MA
OSTI ID:
5406257
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLEOMYCIN
BODY TEMPERATURE
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CESIUM 137
CESIUM ISOTOPES
CHELATING AGENTS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DEDTC
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
HAMSTERS
HYPERTHERMIA
IONIZING RADIATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
TEMPERATURE EFFECTS
TOXICITY
VERTEBRATES
YEARS LIVING RADIOISOTOPES