Light microscopic autoradiography for study of early changes in the distribution of water-soluble materials
- Tsurumi Univ., Yokohama (Japan)
An approach using autoradiography for the study of early changes in the distribution of water-soluble materials and the chemography involved was investigated. Radioactive calcium chloride (45Ca) was injected into the iliac vein of a rat. Ten seconds after the injection the rat was frozen in hexane (-90 degrees C). The frozen rat was embedded in 5% sodium carboxymethyl cellulose and blocked in the coolant. A sheet of plastic tape coated with a synthetic rubber glue was fastened to the trimmed block surface, and whole-body sections 2-10 microns thick were cut with a disposable microtome knife. Selected sections were freeze-dried and then covered with a dried autoradiographic emulsion film about 1 microns thick. The autoradiograph clearly showed the distribution of radioactive calcium in the calcification zone of long bones. The samples chosen to assess chemographic artifacts showed positive and negative chemographies on most of the tissues when these were kept at 23 degrees C, and although both chemographic effects were significantly reduced when the samples were kept at -20 degrees C, cells in several tissues still exhibited positive and negative chemographies. The technique can be used for the study of any animal whose size is suitable for whole-body freeze-sectioning.
- OSTI ID:
- 6072469
- Journal Information:
- Journal of Histochemistry and Cytochemistry; (USA), Journal Name: Journal of Histochemistry and Cytochemistry; (USA) Vol. 38:12; ISSN JHCYA; ISSN 0022-1554
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELEMENTS
EVEN-ODD NUCLEI
HISTOLOGICAL TECHNIQUES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
METALS
MICROSCOPY
NUCLEI
ORGANS
OXYGEN COMPOUNDS
RADIOISOTOPES
RATS
RODENTS
SAMPLE PREPARATION
SKELETON
SOLUBILITY
TISSUE DISTRIBUTION
VEINS
VERTEBRATES
WATER