Localization of calcium in vas deferens using /sup 45/Ca EM autoradiography: relationship to species and the effect of /sup 45/Ca removal
The distribution of calcium was determined in the vas deferens of the guinea pig using /sup 45/Ca electron microscopic autoradiography of rapidly frozen, freeze-dried, and embedded tissue. A selective accumulation of calcium at the plasma membrane and SR was observed in vas deferens that had been incubated in /sup 45/Ca for 65-85 min prior to rapid freezing. Rinsing the tissue in nonradioactive calcium for 6 min prior to rapid freezing significantly altered the distribution of calcium among the plasma membrane, mitochondria, and cytoplasmic matrix. The influence of species on the observed distribution of calcium was also examined. The distribution of calcium in the guinea pig vas deferens was not significantly different from that in the rabbit vas deferens when the tissues were prepared under identical conditions.
- Research Organization:
- Univ. of New Mexico, Albuquerque
- OSTI ID:
- 6476807
- Journal Information:
- Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 4; ISSN ANREA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL VARIABILITY
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELEMENTS
EVEN-ODD NUCLEI
FREEZING
GONADS
GUINEA PIGS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MALE GENITALS
MAMMALS
MEMBRANES
METALS
NUCLEI
ORGANS
RABBITS
RADIOISOTOPES
REMOVAL
RODENTS
TESTES
TISSUE DISTRIBUTION
VERTEBRATES