The distribution of ( sup 14 C)acrylamide in rainbow trout studied by whole-body autoradiography

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Fundamental and Applied Toxicology; (USA)

DOI:https://doi.org/10.1016/0272-0590(90)90233-A· OSTI ID:6967323

Waddell, W J; Lech, J J; Marlowe, C; Kleinow, K M; Friedman, M A ^[1]

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1. Univ. of Louisville, KY (USA)

The distribution of (2,3-{sup 14}C)acrylamide was studied in fingerling rainbow trout by whole-body autoradiography. Fish weighing approximately 7 g were injected ip with 3.2 mg/kg ({sup 14}C)acrylamide (0.1 microCi/g). One group of fish was kept in a fresh flowing water tank and frozen in dry ice/hexane 22 hr after injection; another group was placed in a separate tank of fresh flowing water and frozen 120 hr after treatment. A third group of fish served as nontreated controls. The autoradiographs of the fish at 22 hr show the highest concentration of radioactivity in the kidney, urinary bladder, blood, gallbladder, intestinal contents, and lens of eye. Lesser amounts of radioactivity are seen in the CNS, liver, and gills. Very low concentrations are seen in muscle. By 120 hr the only high concentrations are seen in gallbladder and lens of the eye. Lesser amounts are seen in the sclera, vertebrae, CNS, kidney, wall of intestine, and discrete spots in subcutaneous tissue presumed to be chromatophores. Low amounts are seen in muscle, the tissue usually consumed by man.

• OSTI ID: 6967323
• Journal Information: Fundamental and Applied Toxicology; (USA), Journal Name: Fundamental and Applied Toxicology; (USA) Vol. 14:1; ISSN 0272-0590; ISSN FAATD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRYLAMIDE
AMIDES
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
CONTAMINATION
DISTRIBUTION
FISHES
FOOD CHAINS
LABELLED COMPOUNDS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
TISSUE DISTRIBUTION
TROUT
VERTEBRATES