Subchronic toxicity study of ozonated and ozonated/chlorinated humic acids in Sprague-Dawley rats: A model system for drinking water disinfection

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental Science and Technology; (USA)

DOI:https://doi.org/10.1021/es00013a008· OSTI ID:6056113

Daniel, F B; Robinson, M; Ringhand, H P; Stober, J A ^[1]; Page, N P ^[2]; Olson, G R ^[3]

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1. Environmental Protection Agency, Cincinnati, OH (USA)
2. Page Associates, Gaithersburg, MD (USA)
3. Pathology Associates, West Chester, OH (USA)

Male and female Sprague-Dawley rats were administered drinking water containing humic acids either non-disinfected for following ozonation (O{sub 3}) or ozonation/chlorination (O{sub 3}/Cl{sub 2}) for 90 consecutive days. Test animals drank either of two concentrations of humic acids, 0.25 and 1.0 g/L total organic carbon (TOC), while controls received phosphate-buffered, distilled water. No consistent significant treatment-related effects were observed in body weight gain, organ weights, food or water consumption, or hematological and clinical chemistry parameters. No target organs were identified from the histopathological examination of the tissues. The most significant observation, an increase in liver to body weight ratio for the male animals in the 1.0 g/L O{sub 3}/Cl{sub 2} humic acid group, was not observed in any other group, nor was it corroborated via any biochemical measurements or histopathological analysis. Kidney lesions, primarily chronic progressive nephropathy, were a common observation in both controls and treated groups with no apparent relationship to either humic acid concentration or the disinfection process.

• OSTI ID: 6056113
• Journal Information: Environmental Science and Technology; (USA), Journal Name: Environmental Science and Technology; (USA) Vol. 25:1; ISSN ESTHA; ISSN 0013-936X
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CHEMICAL REACTIONS
CHLORINATION
DATA
DISINFECTANTS
DRINKING WATER
EXPERIMENTAL DATA
GERMICIDES
HALOGENATION
HUMIC ACIDS
HYDROGEN COMPOUNDS
INFORMATION
MAMMALS
MEASURING METHODS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
OZONIZATION
RATS
RODENTS
TOXICITY
VERTEBRATES
WATER