First field trial of an immunoradiometric assay for the detection of malaria sporozoites in mosquitoes
Journal Article
·
· Am. J. Trop. Med. Hyg.; (United States)
OSTI ID:6053530
An immunoradiometric assay (IRMA) using a monoclonal antibody to the major surface protein of Plasmodium falciparum sporozoites was used to assess the P. falciparum sporozoite rate in a West African population of Anopheles gambiae (s.1.). Unlike current dissection techniques, the IRMA could detect sporozoite antigen in dried as well as fresh mosquitoes. In a controlled comparison, the sensitivity of the IRMA was comparable that of the dissection technique. Additionally, the IRMA was species specific and quantitative. Sensitivity of the assay was sufficient to detect sporozoite infections resulting from the development of a single oocyst.
- Research Organization:
- National Inst. of Allergy and Infectious Diseases, Bethesda, MD
- OSTI ID:
- 6053530
- Journal Information:
- Am. J. Trop. Med. Hyg.; (United States), Journal Name: Am. J. Trop. Med. Hyg.; (United States) Vol. 33:4; ISSN AJTHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
ARTHROPODS
DETECTION
DIPTERA
DISEASES
INFECTIOUS DISEASES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LIFE CYCLE
MALARIA
MONOCLONAL ANTIBODIES
MOSQUITOES
PARASITIC DISEASES
RADIOASSAY
RADIOIMMUNOASSAY
SPORES
TRACER TECHNIQUES
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
ARTHROPODS
DETECTION
DIPTERA
DISEASES
INFECTIOUS DISEASES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LIFE CYCLE
MALARIA
MONOCLONAL ANTIBODIES
MOSQUITOES
PARASITIC DISEASES
RADIOASSAY
RADIOIMMUNOASSAY
SPORES
TRACER TECHNIQUES