Novel immunoradiometric assay of thyroglobulin in serum with use of monoclonal antibodies selected for lack of cross-reactivity with autoantibodies

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6280000

Piechaczyk, M; Baldet, L; Pau, B; Bastide, J M

A multisite immunoradiometric assay for measurement of serum thyroglobulin (Tg), designated Magnogel-IRMA-Tg, has been developed, involving magnetic microbeads (Magnogel). This assay is based on the use of five anti-Tg monoclonal antibodies (MAbs) directed against three antigenic regions on the Tg molecule that are not recognized by anti-Tg autoantibodies (aAbs). Four of these MAbs, directed against two antigenic domains, were coupled to the magnetic beads and were used to trap the serum antigen. Another MAb, directed against the third region, was iodinated and served as the labeled second antibody. The Magnogel-IRMA-Tg technique is reproducible, rapid, and sensitive (lower detection limit, 3 micrograms/L). The assay reliably measures serum Tg in the presence of anti-Tg aAbs.

• Research Organization: Unite de Recherche en Immunologie, Montpellier (France)
• OSTI ID: 6280000
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 35:3; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
ANTIGENS
GLOBULINS
IMMUNOASSAY
IMMUNOLOGY
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SENSITIVITY ANALYSIS
SPECIFICITY
THYROGLOBULIN
TRACER TECHNIQUES