Specific immunoradiometric assay of insulin-like growth factor I with use of monoclonal antibodies

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5520830

Scott, M G; Cuca, G C; Petersen, J R; Lyle, L R; Burleigh, B D; Daughaday, W H

We identified two monoclonal antibodies that bind spatially distinct epitopes on insulin-like growth factor I (IGF-I). Using these two antibodies, we developed a simultaneous, two-site immunoradiometric assay (IRMA) specific for IGF-I. This IRMA has no detectable cross reactivity with insulin, proinsulin, prolactin, or somatotropin, and less than 2% crossreactivity with IGF-II. The assay response varies linearly with IGF-I concentrations of 0-800 micrograms/L in serum; the detection limit is about 10 micrograms/L. A comparison of 26 IGF-I serum values from the IRMA and from a previously reported IGF-I specific RIA gave a correlation coefficient of 0.96 with no substantial bias (slope = 1.10). IGF-I values for serum, as an aid in assessing growth abnormalities, are easily (only three pipetting steps) obtained in less than 4 h.

• Research Organization: Mallinckrodt, Inc., St. Louis, MO
• OSTI ID: 5520830
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 33:11; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
GROWTH FACTORS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INSULIN
ISOTOPE APPLICATIONS
MAMMALS
MATHEMATICS
MICE
MITOGENS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REGRESSION ANALYSIS
RODENTS
SPECIFICITY
STATISTICS
TRACER TECHNIQUES
VERTEBRATES