Experimental vaccination of chicks with Plasmodium gallinaceum sporozoites. I. Circumsporozoite proteins are expressed by sporozoites recovered from both salivary glands and midguts of mosquitoes

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · J. Protozool.; (United States)

DOI:https://doi.org/10.1111/j.1550-7408.1987.tb03167.x· OSTI ID:5625221

Daher, V R; Krettli, A U

Immunogenicity of Plasmodium gallinaceum sporozoites for chicks and their in vitro reactivity with normal and specific immune sera were studied. Two sporozoite populations recovered from experimentally infected Aedes fluviatilis were used: sporozoites from salivary glands and sporozoites from midgut oocysts. Populations seven to nine days old of sporozoites recovered from salivary glands were infective for all chicks until the chicks were three weeks old; however, sporozoites recovered from midguts containing oocysts infected these chicks only if isolated on days 8-9, but not on day 7 after the mosquitoes' infective blood meal. Infectivity of the sporozoites was lost after exposure to ultraviolet (UV) light (30 min) or X-rays (13 krad). Inactivated sporozoites from both sources proved highly immunogenic to chicks that were immunized by several intravenous or intramuscular injections. These parasites elicited a strong humoral immune response in the chicks, as measured by the circumsporozoite precipitation (CSP) reaction. The levels of the CSP antibodies were similar with sporozoites from both sources, there being no detectable differences in the percentage of reactive sporozoites or the intensity of the CSP reaction with sera containing antibodies to either sporozoites from salivary glands or sporozoites from oocysts. These results provide the first evidence that avian malaria sporozoites express the circumsporozoite protein that has been extensively characterized in mammalian malaria (rodent, simian, human sporozoites). Furthermore, we observed that the yields of sporozoites obtained from mosquito midguts, on days 8 and 9 of the P. gallinaceum infection, were at least twice as great as those obtained by salivary gland dissection, even 20 days after a blood meal.

• Research Organization: Univ. of Minas Gerais, Fiocruz, Belo Horizonte, Brazil
• OSTI ID: 5625221
• Journal Information: J. Protozool.; (United States), Journal Name: J. Protozool.; (United States) Vol. 34:3; ISSN JPROA
• Country of Publication: United States
• Language: English

Related Subjects

553003* -- Agriculture & Food Technology-- Pest & Disease Control-- (1987-)
60 APPLIED LIFE SCIENCES
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIRDS
BODY
CHICKENS
DISEASES
ELECTROMAGNETIC RADIATION
FOWL
GLANDS
IMMUNE REACTIONS
INFECTIOUS DISEASES
INVERTEBRATES
MALARIA
MICROORGANISMS
ORGANS
PARASITES
PARASITIC DISEASES
PLASMODIUM
PROTOZOA
RADIATION EFFECTS
RADIATIONS
SALIVARY GLANDS
SPOROZOA
ULTRAVIOLET RADIATION
VACCINES
VERTEBRATES