Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11

Kucharska, Iga; Thai, Elaine; Srivastava, Ananya; Rubinstein, John L.; Pomès, Régis; Julien, Jean-Philippe

doi:10.7554/elife.59018

Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11

Journal Article · Mon Nov 30 00:00:00 EST 2020 · eLife

DOI:https://doi.org/10.7554/elife.59018· OSTI ID:1736286

^[1]; ^[2]; Srivastava, Ananya ^[2]; ^[2]; ^[2]; ^[2]

The Hospital for Sick Children Research Inst., Toronto, ON (Canada)
The Hospital for Sick Children Research Inst., Toronto, ON (Canada); Univ. of Toronto, ON (Canada)

Plasmodium sporozoites express circumsporozoite protein (CSP) on their surface, an essential protein that contains central repeating motifs. Antibodies targeting this region can neutralize infection, and the partial efficacy of RTS,S/AS01 – the leading malaria vaccine against P. falciparum (Pf) – has been associated with the humoral response against the repeats. Although structural details of antibody recognition of PfCSP have recently emerged, the molecular basis of antibody-mediated inhibition of other Plasmodium species via CSP binding remains unclear. Here, we analyze the structure and molecular interactions of potent monoclonal antibody (mAb) 3D11 binding to P. berghei CSP (PbCSP) using molecular dynamics simulations, X-ray crystallography, and cryoEM. We reveal that mAb 3D11 can accommodate all subtle variances of the PbCSP repeating motifs, and, upon binding, induces structural ordering of PbCSP through homotypic interactions. Together, our findings uncover common mechanisms of antibody evolution in mammals against the CSP repeats of Plasmodium sporozoites.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357; SC0012704

OSTI ID:: 1736286

Alternate ID(s):: OSTI ID: 1784001

Journal Information:: eLife, Journal Name: eLife Journal Issue: 11, 2020 Vol. 9; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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