Quantitative human chorionic gonadotropin analysis. I. Comparison of an immunoradiometric assay and a radioimmunoassay

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Am. J. Obstet. Gynecol.; (United States)

DOI:https://doi.org/10.1016/S0002-9378(84)80035-6· OSTI ID:6727395

Shapiro, A I; Wu, T F; Ballon, S C; Lamb, E J

An immunoradiometric assay (IRMA) for the quantitative analysis of human chorionic gonadotropin (hCG) was evaluated for specificity, sensitivity, accuracy and precision. The results were compared with those of the conventional radioimmunoassay (RIA) used in our laboratory. The IRMA is a solid-phase, double-antibody immunoassay that sandwiches the intact hCG molecule between the two antibodies. It has specificity, accuracy, and precision which are similar to those of the RIA. The RIA is based upon the assumptions that the antigenicity of the tracer is not altered by the iodination process and that the antibody reacts equally with all of the antigens, including the radiolabeled antigen. The IRMA does not use radiolabeled antigens and thus is free of the assumptions made in the conventional RIA. The IRMA may be more accurate at the lower limits of the assay because it does not require logarithmic transformations. Since the IRMA does not measure the free beta-subunit of hCG, it cannot be endorsed as the sole technique to quantitate hCG in patients with gestational trophoblastic neoplasia until the significance of the free beta-subunit in these patients is determined.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 6727395
• Journal Information: Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Journal Issue: 1 Vol. 148:1; ISSN AJOGA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
COMPARATIVE EVALUATIONS
DIAGNOSIS
DISEASES
GONADOTROPINS
HCG
HORMONES
IMMUNOLOGY
IODINE ISOTOPES
ISOTOPES
MEASURING METHODS
NEOPLASMS
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOLOGY