Theiler's virus RNA and protein synthesis in the central nervous system of demyelinating mice

Journal Article · Mon Jul 15 04:00:00 EDT 1985 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(85)90327-7· OSTI ID:6035470

Cash, E; Chamorro, M; Brahic, M

The authors studied Theiler's virus RNA and capsid protein synthesis in sections of mouse spinal cord using in situ hybridization coupled to immunoperoxidase. They found that the majority of infected cells contain 100 to 500 viral genomes and no detectable capsid antigens. Similarly, baby hamster kidney (BHK) cells, which are permissive to Theiler's virus, do not synthesize capsid if they contain less than 1000 viral genomes. The results demonstrate that virus multiplication is restricted in vivo at the level of RNA replication. They suggest that RNA restriction is sufficient to explain the lack of capsid antigen synthesis.

• Research Organization: Institut Pasteur, Paris, France
• OSTI ID: 6035470
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 1; ISSN VIRLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BIOSYNTHESIS
CENTRAL NERVOUS SYSTEM
DNA
ENZYMES
HAMSTERS
HYBRIDIZATION
LABELLED COMPOUNDS
MAMMALS
MICE
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PROTEINS
RNA
RODENTS
SPINAL CORD
SYNTHESIS
TRANSCRIPTION
TRITIUM COMPOUNDS
VERTEBRATES