RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus

Li, Hui; Zhang, Kaiming; Binzel, Daniel W.; Shlyakhtenko, Lyudmila S.; Lyubchenko, Yuri L.; Chiu, Wah; Guo, Peixuan

doi:10.1080/15476286.2021.1915620

Title: RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus

Journal Article · Thu Apr 29 00:00:00 EDT 2021 · RNA Biology

DOI:https://doi.org/10.1080/15476286.2021.1915620· OSTI ID:1788247

Li, Hui ^[1]; Zhang, Kaiming ^[2]; Binzel, Daniel W. ^[1]; Shlyakhtenko, Lyudmila S. ^[3]; Lyubchenko, Yuri L. ^[3]; Chiu, Wah ^[4];

^[1]

The Ohio State Univ., Columbus, OH (United States). Center for RNA Nanobiotechnology and Nanomedicine, James Comprehensive Cancer Center, and Dorothy M. Davis Heart and Lung Research Institute
Stanford Univ., CA (United States). James H. Clark Center
Univ. of Nebraska Medical Center, Omaha, NE (United States)
Stanford Univ., CA (United States). James H. Clark Center; SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

The quest for artificial RNA viral complexes with authentic structure while being non-replicative is on its way for the development of viral vaccines. RNA viruses contain capsid proteins that interact with the genome during morphogenesis. The sequence and properties of the protein and genome determine the structure of the virus. For example, the Pariacoto virus ssRNA genome assembles into a dodecahedron. Virus-inspired nanotechnology has progressed remarkably due to the unique structural and functional properties of viruses, which can inspire the design of novel nanomaterials. RNA is a programmable biopolymer able to self-assemble sophisticated 3D structures with rich functionalities. RNA dodecahedrons mimicking the Pariacoto virus quasi-icosahedral genome structures were constructed from both native and 2'-F modified RNA oligos. The RNA dodecahedron easily self-assembled using the stable pRNA three-way junction of bacteriophage phi29 as building blocks. The RNA dodecahedron cage was further characterized by cryo-electron microscopy and atomic force microscopy, confirming the spontaneous and homogenous formation of the RNA cage. The reported RNA dodecahedron cage will likely provide further studies on the mechanisms of interaction of the capsid protein with the viral genome while providing a template for further construction of the viral RNA scaffold to add capsid proteins for the assembly of the viral nucleocapsid as a model. Understanding the self-assembly and RNA folding of this RNA cage may offer new insights into the 3D organization of viral RNA genomes. Finally, the reported RNA cage also has the potential to be explored as a novel virus-inspired nanocarrier.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515; U01CA151648; R01EB019036; R01 GM096039; R01 GM118006; P41GM103832; S10 OD021600

OSTI ID:: 1788247

Journal Information:: RNA Biology, Vol. 18, Issue 12; ISSN 1547-6286

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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