Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis
- Department of Biology/Biophysics, Humboldt University Berlin, Invalidenstr. 42, 10115 Berlin (Germany)
- Department of Immunology and Molecular Biology, Vet.-Med. Faculty, Free University Berlin, Philippstr. 13, 10115 Berlin (Germany)
- Max Delbrueck Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin (Germany)
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kindey cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.
- OSTI ID:
- 21141015
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 2 Vol. 377; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
Theiler's virus RNA and protein synthesis in the central nervous system of demyelinating mice
Infection of cells by Sindbis virus at low temperature
Venezuelan equine encephalitis virus entry mechanism requires late endosome formation and resists cell membrane cholesterol depletion
Journal Article
·
Mon Jul 15 00:00:00 EDT 1985
· Virology; (United States)
·
OSTI ID:6035470
Infection of cells by Sindbis virus at low temperature
Journal Article
·
Tue Jun 05 00:00:00 EDT 2007
· Virology
·
OSTI ID:20977033
Venezuelan equine encephalitis virus entry mechanism requires late endosome formation and resists cell membrane cholesterol depletion
Journal Article
·
Mon Apr 10 00:00:00 EDT 2006
· Virology
·
OSTI ID:20779480